Making a Multiple Sequence Alignment in MEGA
This video shows the steps of obtaining nucleotide gene sequences from NCBI, editing the downloaded fasta file, importing the sequences into MEGA, and building a multiple sequence alignment.
MEGA (Molecular Evolutionary Genetics Analysis) is a free software program for working with and analyzing sequences in a phylogenetic framework. You can get it here:
https://www.megasoftware.net/
The citation for it is:
Koichiro Tamura, Glen Stecher, Daniel Peterson, Alan Filipski, and Sudhir Kumar (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution:30 2725-2729.
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