Preparing Tris Buffer

Published: 15 April 2013
on channel: BioNetwork

185,657

1.8k

Tris is a buffer that's used to maintain a stable pH when working with solutions in the lab.
In this video, we'll demonstrate the steps for preparing 250 mL of a 0.5 molar Tris buffer.

We'll measure our powder, mix it with water using a stir plate, and then test our pH with a pH meter. We'll add drops of hydrochloric acid to lower the pH to our target of 7.8.

Check out our free training videos and eLearning tools at http://www.ncbionetwork.org/education....

On this page of the site you can watch the video online Preparing Tris Buffer with a duration of hours minute second in good quality, which was uploaded by the user BioNetwork 15 April 2013, share the link with friends and acquaintances, this video has already been watched 185,657 times on youtube and it was liked by 1.8 thousand viewers. Enjoy your viewing!

318