The Point Spread Function
If you want to understand microscopy, you gotta understand the PSF. The PSF is the basis for many practical and theoretical concepts in light microscopy, both basic and advanced.
Movies focusing on airy disk and 3D reconstruction of PSF were created by Talley Lambert.
Images of cells expressing GFP-tubulin by Talley Lambert.
Image of Abbe statue and diffraction gifs are from Wikipedia.
French subtitles were provided by BioImaging North America.
0:00 Intro
0:24 Definition of resolution
1:39 Close look at a PSF
3:40 PSF results from diffraction & interference
6:16 Diffraction in the microscope
7:09 The effect of the PSF on your images
7:42 Resolution equations
8:54 Rayleigh criterion
9:47 Size of PSF
10:13 Convolution
On this page of the site you can watch the video online The Point Spread Function with a duration of hours minute second in good quality, which was uploaded by the user Microcourses 21 December 2018, share the link with friends and acquaintances, this video has already been watched 71,645 times on youtube and it was liked by 1.6 thousand viewers. Enjoy your viewing!
