341 - Sholl Analysis using python coding
What is Sholl Analysis?
Sholl analysis is a method used in neuroscience to quantify the complexity of neuronal branching patterns. It is performed to measure how neuron dendrites or axons branch out from the cell body.
How is it performed?
A series of concentric circles is overlaid on an image of a neuron.
The circles are centered on the cell body (soma).
The number of times neuronal processes intersect each circle is counted.
When performing automated analysis, the soma is assumed to be the largest, brightest object in the image.
Concentric circles are Placed around the soma because branching typically radiates outward. Usually spaced at regular intervals (e.g., 10 μm apart). Extend far enough to cover the entire dendritic field.
Analysis:
The intersection counts are plotted against circle radius. This plot shows how branching complexity changes with distance from the soma.
This type of analysis is usually performed when ...
Comparing neuron morphology between different cell types or conditions.
Assessing changes in neuronal structure during development or disease.
Image credit:
https://www.mbfbioscience.com/wp-cont...
Code: https://github.com/bnsreenu/python_fo...
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